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Dionex CarboPac PA1 糖和氨基酸色譜柱

更新時間:2019-11-14

簡要描述:

Dionex CarboPac PA1 糖和氨基酸色譜柱
這款的陰離子交換柱與(yu) 脈衝(chong) 電流檢測技術 (PAD) 結合使用,可提供高分辨率的分離。

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Dionex CarboPac PA1 糖和氨基酸色譜柱

對於(yu) 各種基質中單糖、雙糖和線性多糖的測定,使用耐用的 Thermo Scientific? Dionex? CarboPac? PA1 分析和保護柱。 這款的陰離子交換柱與(yu) 脈衝(chong) 電流檢測技術 (PAD) 結合使用,可提供高分辨率的分離。 Dionex CarboPac PA1 樹脂的* Thermo Scientific? Dionex? MicroBead? 薄殼結構,使其在所有緩衝(chong) 鹽溶液中可保持 pH 0–14 的穩定性。 薄殼的樹脂結構提供出色的質量傳(chuan) 遞優(you) 秀的傳(chuan) 質,從(cong) 而實現快速的梯度再平衡。

 

 

描述

用於(yu) 碳水化合物分離的耐用型柱

  • 經驗證適用於(yu) 各種正式的食物分析方法

  • 特別適合針對營養(yang) 標簽進行的食物分析

  • 非常適用於(yu) 中性與(yu) 酸性單糖的分離

  • 靈敏度低於(yu) Dionex CarboPac PA10 或 PA20 柱

  • 提供微孔、標準孔和半製備規格

關(guan) 於(yu) Dionex CarboPac 柱係列

Dionex CarboPac 係列提供精選的高性能伟德苹果手机app下载安装包 (HPLC) 柱,每一款均為(wei) 不同類別化合物而優(you) 化。 與(yu) 能夠檢測碳水化合物而不產(chan) 生衍生物的 PAD 配合使用,這些色譜柱支持簡單可靠的糖醇、單糖和雙糖、多糖和低聚糖分離技術。

Dionex CarboPac 柱提供以下成分的高分辨率分離:

  • 膳食纖維中的糖蛋白單糖和複合碳水化合物,包括果聚糖、麥芽糊精和支鏈澱粉

  • 單糖,例如從(cong) 糖蛋白水解物和植物水解物得到的單糖

  • 單糖和雙糖

 

用途和特點
AminoPac PA1柱用於(yu) 氨基酸的快速分離。特別適用於(yu) 分析酸性和酸不穩定性氨基酸,以及用陽離子交換分離不*的氨基酸對。

 

Dionex CarboPac PA1 糖和氨基酸色譜柱

訂貨信息:

Dionex CarboPac PA1 糖和氨基酸分析柱

分析柱
CarboPac PA1 Analytical Column (2 x 250 mm)057178
CarboPac PA1 Analytical Column (4 X 250 mm)035391
CarboPac PA1 Semi-Preparative Column (9 x 250 mm)039686
CarboPac PA1 Semi-Preparative Column (22 x 250 mm)0SP2866
保護柱
CarboPac PA1 Guard Column (2 x 50 mm)057179
CarboPac PA1 Guard Column (4 x 50 mm)043096
CarboPac PA1 Guard (9 x 50 mm)063501

 

 

 

Ideal Column for the Separation of Neutral and Acidic Monosaccharides

The CarboPac? PA1 is an anion-exchange column for the separation of mono-, disaccharides, and specific oligosaccharides using an isocratic eluent. Sialic acid analysis has also been demonstrated on this column. 

  • General-purpose column for isocratic determination of monosaccharides and disaccharides

  • Detection by pulsed amperometric detection; no derivatization needed

  • Approved for use in a variety of official methods for the analysis of foods

The unique MicroBead? pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration.

The CarboPac PA1 column is used with pulsed amperometric detection; no derivatization is required. Sensitivity on the PA1 is not as high as with the CarboPac PA20 or PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods.

 

 

CarboPac PA1 Column for Routine Carbohydrate Analysis  Specifications
DimensionsCarboPac PA1 Analytical Column:
4 × 250 mm and 2 × 250 mm
CarboPac PA1 Guard Column:
4 × 50 mm and 2 × 50 mm
CarboPac PA1 Semipreparative Column:
22 × 250 mm
Maximum operating pressure4000 psi (27.9 MPa)
Temperature range4–55 °C
Recommended operating temperature: ambient
Typical operating conditions1500 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min (for 4 × 250 mm)
mobiles phase compatibilitypH 0–14; up to 2% of common HPLC solvents.
Acetate or hydroxide eluents only.
Resin composition10-μm-diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500-nm MicroBead quaternary ammonium functionalized latex (5% cross-linked).
Anion-exchange capacityApproximately 100 μeq/column (4 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded-ferrule-style end fittings.
All components are nonmetallic.

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